Somayeh Zeinali; Mohammad Amir Karimi Torshizi; Farid Shariatmadari
Volume 25, Issue 2 , July 2023, , Pages 201-213
Abstract
Introduction Bacteria of the intestinal tract may have a profound impact on lipids' digestion and utilization. The proposed mechanism for reduced lipids digestibility is the de-conjugation of bile salts, via bacterial bile salt hydrolase (BSH) enzyme activity. The activity of BSH is well recognized in ...
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Introduction Bacteria of the intestinal tract may have a profound impact on lipids' digestion and utilization. The proposed mechanism for reduced lipids digestibility is the de-conjugation of bile salts, via bacterial bile salt hydrolase (BSH) enzyme activity. The activity of BSH is well recognized in some genera of lactic acid bacteria like Enterococci. Bacteriophages are recognized as bacteria-eating viruses, which are host specific in contrast to antibiotics. We find bacteriophages that lysis the laying hen's intestinal Enterococci. It is expected that oral administration of bacteriophage lessens the negative impact of Enterococci BSH activity on lipids digestion.
Materials and Methods A total of 240 high-line W-36 laying hens aged 50 weeks were tested in six treatments with five replications and eight pieces in each replication for eight weeks in a completely randomized design. Treatments include, 1) control (basal diet without additives), 2) basal diet + antibiotic (virginiamycin), 3) basal diet + lipid-lowering drug (atorvastatin), 4) basal diet + bile salt powder, 5) basal diet + oral gavage of Enterococcus bacteria, and 6) basal diet + oral gavage of bacteriophage against Enterococcus.
Results and Discussion The results showed that the group receiving the lipid-lowering drug (atorvastatin) had significantly the lowest percentage of egg production, the lowest egg mass, and the highest feed conversion rate (P<0.05). Enterococcus bacteria and atorvastatin had the same performance, although Enterococcus bacteria did not increase the feed conversion rate as much as the lipid- atorvastatin, it performed poorly compared to the bacteriophage. The use of phage in poultry improved performance. No significant effect among treatments was observed in albumin, glucose, uric acid, calcium, and phosphorus. The level of cholesterol, triglyceride, total protein, and globulin in the atorvastatin group was lower compared to the antibiotic and bile salt groups (P<0.05). Cholesterol and triglyceride levels were higher in the antibiotic, bacteriophage, and bile salt powder treatments than in the other treatments (P<0.05). The lowest concentration of uric acid was observed in the control group and the highest percentage of hematocrit was observed in the bacteriophage and control groups. The role of antibiotic and bile salt in the rest of the reports was the same as bacteriophage but regarding the increase of hematocrit in bacteriophage treatment, phages showed their superiority in this field (P<0.05). The total number of aerobic bacteria, lactic acid bacteria, enterococcus, and coliforms in the bacteriophage group was less than in the bacteria group (P<0.05). However, the digestibility of crude protein was not significantly different between bacteriophage and bacteria groups (P<0.05). The total population of aerobic bacteria was lower in antibiotic and bacteriophage treatments. This study demonstrated the ability to use bacteriophage to reduce the population of specific bacteria. The population of lactic acid bacteria was higher than other treatments in bacteria treatment. Bacteriophage had been more effective to reduce the population of Enterococcus bacteria than other treatments. A close competition between antibiotics and bacteriophage was observed. Regarding the reduction of the E.coli bacteria population, it showed the appropriate ability of bacteriophage to replace the antibiotics. The digestibility of ether extract and dry matter in the bacteriophage was higher than in the bacteria group. The dry matter digestibility in antibiotic and bacteriophage is similar, but bile salt powder was not similar to bacteriophage in this case, and the dry matter in bile salt powder was the lowest (P<0.05).
Conclusion Based on the results, oral bacteriophage could improve dry matter digestibility and reduce the Enterococcus bacteria population without a significant impact on production performance in laying hens.
Saifali Varmaghany; mohammad amir karimi turshizi; hoshang lotfulahian; hosang jafari; Mohammad Hossein nemati
Volume 20, Issue 1 , May 2018, , Pages 69-82
Abstract
In order to investigate the effect of fresh whole garlic bulb (FGBP) on production index, immune system and carcass characteristics of Arian broiler strain, 592 male one day old chicks were studied during 42 days in two independent experiments. The first experiment was conducted under normal temperature ...
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In order to investigate the effect of fresh whole garlic bulb (FGBP) on production index, immune system and carcass characteristics of Arian broiler strain, 592 male one day old chicks were studied during 42 days in two independent experiments. The first experiment was conducted under normal temperature condition (NTC) with 4 treatments, 4 replicates and 21 birds per replicate and second experiment was conducted under cold temperature condition (CTC) with 4 treatments, 4 replicates and 16 birds per replicate basis balanced completely randomized design. The treatments were 1- basal diet without FGBP (control group), 2, 3 and 4 basal diet containing 0.5, 1 and 1.5 percent FGBP, respectively under both NTC and CTC. The highest level of body weight gain and production index were related to treatment of basal diet with 0.5 percent FGBP treatment under both NTC and CTC (P
abbas ashoori; Alireza Behnamifar
Volume 19, Issue 3 , November 2017, , Pages 685-696
Abstract
The effects of the herbal extracts (Rosemary officinalis L., Salvia officinalis), propolis, virginiamycin antibiotic and the herbal coccidiostat were studied on broiler chickens challenged with two E. coli strains (O78K80 & O2K12). A total of 476 female cobb 500 broilers was allocated in a completely ...
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The effects of the herbal extracts (Rosemary officinalis L., Salvia officinalis), propolis, virginiamycin antibiotic and the herbal coccidiostat were studied on broiler chickens challenged with two E. coli strains (O78K80 & O2K12). A total of 476 female cobb 500 broilers was allocated in a completely randomized design considering seven treatments with four replicates and 17 birds per each for 42 days. Negative and positive control groups received a basal diet with no supplementation. The two herbal groups received a basal diet with 1 mL of ethanol herbal extracts (Rosemarinus officinalis & Salvia officinalis) / L drinking water, respectively. The virginiamycin (10%), the herbal coccidiostat and the ethanol extract of propolis groups fed the basal diet plus 150, 200 and 500 mg / kg diet, respectively. All groups except negative control challenged with two E. coli strains (O78K80 & O2K12) in 14d of rearing period. Herbal extract decreased the intestinal population of E. coli and Salmonella compared to the positive control (P<0.05). In the rearing period, feed conversion ratio and feed intake in chickens received herbal extracts and herbal coccidiostat were significantly lower than control groups (P<0.05). Birds that received rosemary and propolis had the lowest relative weight of the breast compared to other treatments (P<0.05).Herbal extracts decreased the cholesterol and increased HDL levels of serum (P<0.05). It could be concluded that the herbal extracts can be used as an alternative to antibiotics as growth promoters in broiler chicks.
Razieh Badiefar; Farid Shariatmadari
Volume 18, Issue 4 , December 2016, , Pages 789-799
Abstract
The aim of this study was to investigate the influence of whole barley with GalyZym poly enzymesupplementation on performance and nutrient digestibility in laying hens. In this study 105 laying hens (w-36) at the age of 40 to 52 weeks were distributed randomly in 5 treatments, with 7 replication, and ...
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The aim of this study was to investigate the influence of whole barley with GalyZym poly enzymesupplementation on performance and nutrient digestibility in laying hens. In this study 105 laying hens (w-36) at the age of 40 to 52 weeks were distributed randomly in 5 treatments, with 7 replication, and 3 birds in each. The experimental treatments included control diet, diets containing barley seed without enzyme, diets containing barley and Galyzym enzyme supplementation,diet containing chopped oatmeal without enzyme, diet containing chopped oatmeal and Galyzym enzyme supplementation. Effects of treatments on production and egg weight was not significant, But laying hens that fed a diet containing barley seeds (with or without enzyme supplementation), had more daily feed intake compared to the control birds (P<0.05). Effects of treatments on egg quality characteristics were not significant. Addition of Galyzym enzyme to the diets containing barley had no effect on apparent metabolizable energy of the diet, dry matter and Organic matter digestibility as well. When enzyme was added to the diets containing oatmeal,an increase of protein digestibility were observed.According toresults of this study, 50 percent of corn in corn and soybean meal based diets on can be replaced with barley grain supplemented with enzyme, without decreasing the performances and digestibility of the nutrients.
Ali Reza Amouyan Khaveh; Mohammad Amir Karimi Torshizi; Farid Shariatmadari
Volume 18, Issue 1 , April 2016, , Pages 85-94
Abstract
Effect of intermittent mash-pellet feeding programs on broilers performance, ascites susceptibility and economic yield was studied using 480 broilers in six treatments and five replicates of 16 chicks per pen were used in a completely randomized design. Treatments including: 1. Mash feed, 2. Heated-mash ...
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Effect of intermittent mash-pellet feeding programs on broilers performance, ascites susceptibility and economic yield was studied using 480 broilers in six treatments and five replicates of 16 chicks per pen were used in a completely randomized design. Treatments including: 1. Mash feed, 2. Heated-mash feed, 3. Mash feed in starter and grower and pellet feed in finisher, 4. Mash feed in starter and pellet feed in grower and finisher, 5. Pellet feed, 6. Grinded-pellet feed. Pellet feeding increased the feed intake and body weight gain and improved the feed conversion ratio, although the ascites susceptibility increased (P<0.01). Mash feeds decreased the body weight gain and increased the feed conversion ratio (P<0.01). Feeding pellet in finisher resulted in the same feed conversion ratio with feeding pellet for whole period or grower and finisher periods, however feed conversion ratio in pellet fed birds was better than mash fed birds (P<0.01). Feeding pellet for whole period is necessary to obtain maximum feed intake and body weight gain. Feeding mash feed in the first four weeks or whole period reduced the ascites susceptibility (P<0.01). The ascites susceptibility increased by increase in pellet feeding length (P<0.01). Feeding pellet diets reduced the feed cost for a kg live weight production and the increased gross profit compared to mash diets (P<0.05). In order to use the benefits of pellet feed and avoid high mortality rates and ascites susceptibility, it is recommended to start with mash feed and fed pellet in growing and finisher periods.
Jalil Noori Raygani; Mohammad Amir Karimi Torshizi; Shaban Rahimi; Omid Madadgar
Volume 17, Issue 2 , October 2015, , Pages 199-209
Abstract
Three experiments were carried out for isolation of bacteriophage against growth reducing Enterococcus faecalis and evaluation of its efficacy to control Enterococci colonization in intestine of Japanese quails. In experiment one, 60 Enterococci spp were isolated from gastrointestinal contents of adult ...
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Three experiments were carried out for isolation of bacteriophage against growth reducing Enterococcus faecalis and evaluation of its efficacy to control Enterococci colonization in intestine of Japanese quails. In experiment one, 60 Enterococci spp were isolated from gastrointestinal contents of adult quails, which among them seven isolates were selected based on preliminary tests. In experiment two, effect of oral challenge by the seven selected isolates along with Enterococcus faecalis ATCC51299- as positive control on growth performance of growing quails was evaluated. In experiment three, lytic bacteriophage was screened against Enterococcus faecalis using quails excreta and poultry house swage as a sources of phage. Finally, efficiency of bacteriophage therapy (104 pfu/mL) in biocontrol of enterococcal colonization of intestine was evaluated in quails orally challenged by Enterococcus faecalis (107 cfu/mL). Among seven isolates from the first experiment, an isolate which reduced the growth of growing quails and had the minimum mortality in comparison to negative control group (P<0.05) was final selected and identified at the species level using biochemical tests based of carbohydrates fermentation profile as Enterococcus faecalis. Oral administration of isolated bacteriophage against Enterococcus faecalis was able to reduce the intestinal enterococci colonization to a level comparable to negative control group (P<0.05). In conclusion, isolated bacteriophage against Enterococcus faecalis effectively reduced enterococci colonization in intestinal content of quails and in this way, a growth reducing factor in poultry could be biocontrolled.
Hamid Reza Khajavi; Mohammad Amir Torshizi; Hamed Ahmadi
Volume 16, Issue 2 , October 2015, , Pages 113-122
Abstract
In order to evaluate the effect of feeding different levels of vermi-humus supplement (as a source of humic acid) on the performance of broiler chickens an experiment was conducted using 240 broiler chickens (Ross 308) in a completely randomized design by four treatments (vermi-humus levels of 0, 0.5, ...
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In order to evaluate the effect of feeding different levels of vermi-humus supplement (as a source of humic acid) on the performance of broiler chickens an experiment was conducted using 240 broiler chickens (Ross 308) in a completely randomized design by four treatments (vermi-humus levels of 0, 0.5, 1 and 1.5 percent) and five replicates of 12 chickens per each for 42 day. The experimental treatments had no significant effect on the growth performance, carcass percentage, internal organs weights, serum concentrations of calcium and phosphorus and tibia bone ash. Increasing the levels of vermi-humus up to one percent of diet resulted in linear decline in pH of breast meat (P<0.05), although the level of 1.5 percent increased the pH value. The thigh meat pH increased at 1 and 1.5 percent levels of vermi-humus supplementation in diet compared to control and 0.5 percent groups. The concentration of malon dialdehyde of fresh and preserved thigh meat decreased linearly with increasing the levels of vermi-humus supplementation in diet, while the malon dialdehyde of breast meat changed in quadratic manner (P<0.01). Water holding capacity of meat increased linearly with enhancement of level of vermi-humus supplement in the diet (P<0.01). Addition of vermi-humus at the level of one percent in diet leads to improvements in the color, smell, juiciness and overall acceptance of breast meat compared to the control (P<0.05), but these parameters had not affected in thigh meat. The results showed that feeding vermi-humus up to one percent of diet could improve the breast meat quality, taste and flavor without adverse effect on performance.
Daryush Khademi Shurmasti; Farid Shariatmadari; Mohammad Amir Karimi Torshizi; Houshang Lotfollahian
Volume 17, Issue 2 , October 2015, , Pages 211-221
Abstract
The present study was carried out to evaluate the effects of vegetable fat sources and additives on performance and morphological parameters of the small intestine of Arain broiler chickens. Birds were randomly used in a 2×3 factorial arrangement of dietary treatments included two fat sources (canola ...
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The present study was carried out to evaluate the effects of vegetable fat sources and additives on performance and morphological parameters of the small intestine of Arain broiler chickens. Birds were randomly used in a 2×3 factorial arrangement of dietary treatments included two fat sources (canola or pumpkin seed) and three additives (without additive, 0.1 g/kg probiotic and 0.15 g/kg virginamycin) with four pen replicates and 20 birds per each. The growth performance and productive traits, with using of neither additives nor fat sources or fat sources × additives were unaffected. However, probiotic was significantly decreased crypt depth and increased villi height to crypt depth ratio in jejunum and ileum (P<0.01). Likewise, the highest of villi height and villi height to crypt depth ratio in jejunum and ileum especially was obtained with combination of pumpkin seed oil and probiotic (P<0.05). Crypt depth, villi thickness and surface area was higher in duodenum of broilers were fed with pumpkin seed oil (P<0.05).Thus, probiotic bactocell can be used in diets contain linoleic-oleic (pumpkin seed oil) and linolenic (canola oil) fat groups without negative effect on performance and productive traits.
Seyed Naser Khaleghi Miran; Mohammad Amir Karimi Torshizi; Shaban Rahimi; Mohammad Reza Bassami; Sakine Babaei
Volume 17, Issue 2 , October 2015, , Pages 235-245
Abstract
The effect of ethanolic extract of Iranian green propolis, rosemary and earthworm meal to determine on performance, immune system and blood metabolites of Japanese quail, using 160 mixed-sex quail chicks in a completely randomized design by four treatments (including ethanolic extract of propolis 0.1 ...
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The effect of ethanolic extract of Iranian green propolis, rosemary and earthworm meal to determine on performance, immune system and blood metabolites of Japanese quail, using 160 mixed-sex quail chicks in a completely randomized design by four treatments (including ethanolic extract of propolis 0.1 percent, rosemary leaves meal 0.5 percent, earthworm meal 0.5 percent and control) and four replicates of 10 birds in each replication for 42 days. Earthworm meal improved the performance (P<0.05). The treatments had no significant effect on the antibody production against sheep red blood cells. The highest antibody titers against Avian Influenza and Newcastle disease were related to rosemary and propolis, respectively (P<0.05). Rosemary supplemented group showed a better response to cell mediated immunity (P<0.05). Addition of propolis and rosemary in diet led to improvement in blood metabolites and earthworm meal decreased blood serum triglycerides, cholesterol and low density lipoproteins compared to the control treatment (P<0.05). The results showed that propolis and rosemary enhanced immunity. Furthermore, they were reduced serum glucose and lipids. Although earthworm meal improved performance but it was less effective in improving the immune system and blood metabolites.
Sakineh Babaei; Shaban Rahimi; Mohammad Amir Karimi Torshizi; Gholam Hosein Tahmasebi; Seyed Naser Khaleghi Miran
Volume 17, Issue 2 , October 2015, , Pages 311-320
Abstract
The effect of honey, royal jelly and bee pollenon performance, immune system and blood factors of Japanese quail, using 160 quail chicks in a completely randomized design by four treatments ]control (without additives), pollen powder 1 g/kg, royal jelly 125 ppm/L, honey 22 g/L) and four replicates of ...
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The effect of honey, royal jelly and bee pollenon performance, immune system and blood factors of Japanese quail, using 160 quail chicks in a completely randomized design by four treatments ]control (without additives), pollen powder 1 g/kg, royal jelly 125 ppm/L, honey 22 g/L) and four replicates of 10 chicks in each replication for 42 days. The experimental groups had no significant effect on feed intake and mortality rate (P>0.05) in whole period. Birds that received honey in drinking water, the best daily body weight gain and feed conversion ratio had compared to other groups. The experimental groups had no significant effect on the antibody production against sheep red blood cells. The highest antibody titers against Newcastle disease and Avian Influenza viruses were related to honey treatment (P<0.05). Addition of pollen to diet, royal jelly and honey in drinking water were significantly affected spleen weight compared to control group (P<0.01). Honey and pollen showed a better response to cell mediated immunity (P<0.01). The use of honey bee products improved blood factors (P<0.01). The results showed that the honey and royal jelly enhanced immunity and performance of the Japanese quails. Furthermore, they were reduced serum glucose and lipids in these birds. Although bee pollenimproved blood factors but it was less effective in improving the immune system and performance.